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SHR Neuro Krebs Kardio Lipid

Mayrhauser, U; Stiegler, P; Stadlbauer, V; Koestenbauer, S; Leber, B; Konrad, K; Iberer, F; Tscheliessnigg, K.
Cell to cell interactions influence sensitivity of liver cell lines during hyperthermia.
Anticancer Res. 2011; 31(11):3713-3717
Web of Science PubMed

 

Autor/innen der Med Uni Graz:
Iberer Florian
Konrad Katja Alena
Köstenbauer Sonja
Leber Bettina
Mayrhauser Ursula
Stadlbauer-Köllner Vanessa
Stiegler Philipp
Tscheliessnigg Karlheinz
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Abstract:
Background: Thermal cancer therapy is used for hepatocellular carcinoma treatment. In this study we investigated the effect of hyperthermia on liver cells and compared data of our different cell culture fibrosis models (transwell vs. co-culture model). Materials and Methods: The cell lines HepG2 and LX-1 were seeded in different numbers in transwells to simulate different grades of fibrosis and then heated from 55 degrees C to 85 degrees C for different time spans. Thereafter, metabolic activity was measured. Results: Heating at 65 degrees C showed that the greater the number of LX-1 cells treated together with HepG2 cells the lower the metabolic activity of HepG2 cells was. Compared to our previous co-culture study, there were significantly different results in cell survival from 55 degrees C to 75 degrees C. Conclusion: The co-culture fibrosis model is more physiological than the transwell model because it allows a higher seeding density and a higher degree of cell to cell interactions. Therefore, it is more efficient for investigating the effect of hyperthermia on liver cells.
Find related publications in this database (using NLM MeSH Indexing)
Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - therapy
Cell Communication -
Cell Proliferation -
Cells, Cultured -
Coculture Techniques -
Fibrosis - metabolism Fibrosis - pathology
Hepatic Stellate Cells - metabolism Hepatic Stellate Cells - pathology
Humans -
Hyperthermia, Induced -
Liver Neoplasms - metabolism Liver Neoplasms - pathology Liver Neoplasms - therapy

Find related publications in this database (Keywords)
Hyperthermia
thermal cancer therapy
hepatic stellate cells
fibrosis
HCC
transwell
co-culture
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